Antimicrobial Evaluation of Hibiscus rosa-sinensis plant extracts against Some Pathogenic Bacteria
In the present study, five different cultivars of Hibiscus rosa-sinensis were studied for antibacterial activity against pathogenic bacteria. Antimicrobial activity of 4 different extracts (Hexane, Ethyl acetate, Methanol and Distilled water) of Hibiscus rosa-sinensis Were tested against Gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, Streptomyces alboniger, Micrococcus luteus, Staphylococcus epidermis) and Gram negative bacteria(Pseudomonas aeruginosa, Bordetella bronchiseptica). Antibacterial activity was carried out by agar diffusion method. Ethyl acetate leaves extract showed significant antibacterial activity against ‘ORANGE’ cultivar. Out of these four plant extract, tested ethyl acetate and methanol extract showed good antimicrobial activity than the other extract and very less antimicrobial activity was observed by the Hexane extract. Minimum inhibitory concentration (MIC) of ethyl acetate extract was screened against tested bacteria. It showed inhibitory action at conc. 0.625 mg ml–1. Out of four different extracts, methanolic extract was proven to be most suitable solvent for extraction and antimicrobial activity.