Antimicrobial Evaluation of Hibiscus rosa-sinensis plant extracts against Some Pathogenic Bacteria

  • Reena Patel Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New V.V.Nagar, 388 121, Gujarat, India
  • Aditi Patel Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidyanagar, Anand, Sardar Patel University, Anand, India
  • Dharmesh Vaghasiya Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New V.V.Nagar, 388 121, Gujarat, India
  • Anju Nagee Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New V.V.Nagar, 388 121, Gujarat, India

Abstract

In the present study, five different cultivars of Hibiscus rosa-sinensis were studied for antibacterial activity against pathogenic bacteria. Antimicrobial activity of 4 different extracts (Hexane, Ethyl acetate, Methanol and Distilled water) of Hibiscus rosa-sinensis Were tested against Gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, Streptomyces alboniger, Micrococcus luteus, Staphylococcus epidermis) and Gram negative bacteria(Pseudomonas aeruginosa, Bordetella bronchiseptica). Antibacterial activity was carried out by agar diffusion method. Ethyl acetate leaves extract showed significant antibacterial activity against ‘ORANGE’ cultivar. Out of these four plant extract, tested ethyl acetate and methanol extract showed good antimicrobial activity than the other extract and very less antimicrobial activity was observed by the Hexane extract. Minimum inhibitory concentration (MIC) of ethyl acetate extract was screened against tested bacteria. It showed inhibitory action at conc. 0.625 mg ml–1. Out of four different extracts, methanolic extract was proven to be most suitable solvent for extraction and antimicrobial activity.

Published
2012-11-29
How to Cite
PATEL, Reena et al. Antimicrobial Evaluation of Hibiscus rosa-sinensis plant extracts against Some Pathogenic Bacteria. Bulletin of Environmental and Scientific Research, [S.l.], v. 1, n. 3-4, p. 14-17, nov. 2012. ISSN 2278-5205. Available at: <http://besr.org.in/index.php/besr/article/view/30>. Date accessed: 23 sep. 2020.
Section
Articles