Factors affecting cellulase production by Aspergillus fumigatus SK1 from solid state fermentation of oil palm empty fruit bunches using application of 2-level factorial design

  • Mohammadreza Soleimaninanadegani
  • M S Madihah
  • S K Ang

Abstract

A study was conducted to screen parameters affecting
the production of cellulase by Aspergillus fumigatus
SK1 on solid state fermentation of oil palm empty fruit
bunch using the application of 2-level factorial design.
Through the two- Level full Factorial Design (2LFD), the
experimental design was employed to screen the significant
environmental factors for cellulase (CMCase,
FPase and ¯-glucosidase) production. Full factorial design
of four factors with six center point was conducted
which consist of 22 runs. All data obtained were then
used as input to the Design Expert software for further
analysis, according to steps outline. ANOVA analysis
showed that the models were significant (p < 0.0001)
for CMCase, FPase and ¯-glucosidase. Inoculum size,
temperature, moisture and ammonium sulphate were
identified as significant factor affecting cellulase production
on solid state fermentation. Statistical analysis
revealed that the linear model is significant with R2
value of 0.9569, 0.9867 and 0.9873 for CMCase, FPase
and ¯-glucosidase production, respectively. Cellulase
production was found to be high at the optimal condition
were: inoculums size 109, moisture 74%, temperature
39 and ammonium sulphate 3.5 g/L. The obtained
optimal process parameters predicted and verified by
confirmation experiments with less than 5% error.

Published
2014-07-24
How to Cite
SOLEIMANINANADEGANI, Mohammadreza; MADIHAH, M S; ANG, S K. Factors affecting cellulase production by Aspergillus fumigatus SK1 from solid state fermentation of oil palm empty fruit bunches using application of 2-level factorial design. Bulletin of Environmental and Scientific Research, [S.l.], v. 3, n. 2-3, p. 16-24, july 2014. ISSN 2278-5205. Available at: <http://besr.org.in/index.php/besr/article/view/67>. Date accessed: 17 sep. 2019.
Section
Articles